Not Released
Sample In stock
Experience: Works
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Measurement

Part:BBa_K516130:Design

Designed by: Susanna Zucca, Federica Sampietro, Giuseppe Bertoni, Paolo Magni   Group: iGEM11_UNIPV-Pavia   (2011-09-08)

Constitutive promoter [J23101] with mRFP, RBS B0030


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly.

Source

Registry

References


  • Kelly JR, Rubin AJ, Davis JH et al. (2009) Measuring the activity of BioBrick promoters using an in vivo reference standard. J. Biol. Eng. 3:4.
  • Salis HM, Mirsky EA, Voight CA (2009)Automated design of synthetic ribosome binding sites to control protein expression. Nat. Biotechnol.27:946-950.